A Survey on Nuclear-to-cytoplasmic Ratio Analysis Using Image Segmentation

In the Bio-medical environment, for the analysis of abnormality detection in the skin including skin cancer in the epidermal layer, the traditional method used is biopsy procedure. The obtained biopsy specimens are processed by various methods viz, various orders of harmonic generations enumerate certain results resulting in the chances of some unexpected infections. Later on to overcome these infections, the followed is optical virtual biopsy procedure with higher harmonics. This yields better results than before. The watershed transform with its gradient works more accurate on the virtual specimens which is observed by microscopically with higher penetration depth.